ASPCR
Alternative splicing analysis by end-point RT-PCR with capillary electrophoresis quantification.
Alternative splicing analysis by end-point RT-PCR, or ASPCR, involves the relative quantification of alternative isoforms from a RT-PCR reaction. We design primers to amplify both isoforms in the same reaction tube. The splicing event is thus quantified by capillary electrophoresis from the ratio of the longer isoform (inclusion) against the sum of the shorter (exclusion) and longer isoforms. This ratio is dubbed "percent splicing index", or PSI. PSI data is presented in bar graphs, heatmaps and data sheets.
Key Features
Fragment Analyzer 5200
Capillary electrophoresis system by Agilent for precise fragment analysis.
Optimal Detection Range
Detection and quantification for 80 to 650 bps amplicons with at least 15% size separation.
High Throughput
2,500 reactions daily throughput for fast turnaround.
Available Applications
Alternative Splicing Analysis
Alternative splicing event quantification analysis from selected events or from gene splicing survey.
RNA-Seq Validation
RNA-Seq splicing validation by RT-PCR.
Bacterial Strain Typing
Bacterial molecular strain typing by ribotyping and multi-locus variable-number tandem repeat analysis (MLVA).
Pricing
| Number of Reactions | UdeS | Canadian Academic |
|---|---|---|
| 1–24 | $12.00 | $14.64 |
| 25–48 | $5.00 | $6.10 |
| 49–72 | $4.00 | $4.88 |
| 73+ | $3.00 | $3.66 |
- For academics external to Canada and industrials please contact us for an accurate quote.
- Not including cost for sample preparation and PCR primer.
- Prices subject to change.
Ready to Start Your Project?
Contact us today to discuss how our ASPCR services can support your research.