The RNomics Platform at Universit√© de Sherbrooke provides a wide range of PCR, NGS, and bioinformatics services to the research community. We have developed an automated computational and experimental platform for large scale PCR-based investigations. Primary applications are for comparative gene expression studies using SYBR green and dual fluorescent probe based quantitative PCR (QPCR), and the annotation and analysis of alternative splicing using endpoint PCR coupled with microcapillary electrophoresis (ASPCR). We routinely design, execute and analyze thousands of QPCR and ASPCR reactions per day. Associated services include RNA and DNA isolation and quality control from cell culture and tissue sources, and extensive bioinformatics support for experimental design and data analysis. Clients can access their results and all experimental data via a secure web portal, where several computational modules are available to optimize data analysis and presentation. The Platform's expert staff is also available for direct consultation at all stages of each project. The Platform is continually developing new assays and bioinformatics protocols to meet the needs of its expanding client base.

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