qRT-PCR
qRT-PCR
The qRT-PCR service is a complete gene expression assay workflow from target selection, primer design and validation, to qRT-PCR run, and relative expression analysis.
- Quantitative RT-PCR using SYBRgreen fluorescence technology.
- Bio-Rad CFX RealTime System: 384-plate (three units) and 96-plate (one unit).
- Primer validation using standard curve and target specificity verification by capillary electrophoresis.
- 5000 reactions daily throughput.
- Growing database of pre-validated primers (see here).
Applications
- Gene expression using qBase and Genorm methods (Hellemans 2007; Vandesompele 2002). Expression targets include: Global, isoform-specific, sequence-specific or non-coding RNA.
- miRNA expression using stem-loop method (Chen 2005)
- Telomere and TREC DNA quantification using Dion et al. method
| Rates CAD$ | ||
| Number of reactions | UdeS | Canadian academic |
| 1-75 | 5.15 | 7.73 |
| 76-100 | 4.35 | 6.53 |
| 101-150 | 3.25 | 4.88 |
| 151-200 | 2.50 | 3.75 |
| 201-250 | 2.00 | 3.00 |
| 251-350 | 1.50 | 2.25 |
| 350+ | 1.25 | 1.88 |
- For academics external to Canada and industrials please contact us for an accurate quote.
- Not including cost for sample preparation and PCR primer and hydrolysis probe design if applicable.
- Prices subject to change.