Plasmid sequencing
Plasmid sequencing
Using a third-generation sequencing method (Oxford Nanopore Technologies (ONT)), our service enables high-quality sequencing of your whole plasmid(s) at an affordable price ($25 CAD/plasmid).
This service is for you if:
- You have a long insert to sequence (requiring several Sanger sequencing reactions).
- You own or have received a plasmid for which you do not know sequence.
- You want to check that a plasmid you've been using for a long time doesn't have unexpected mutations in its backbone.
This service may be less suitable for you if:
- You're only interested in knowing the sequence of a small plasmid insert.
We now perform a sequencing run every Thursday, ensuring a more predictable turnaround time. Samples received by Wednesday will be included in the next run. Contact us if you have any questions about scheduling.
- Does not need specific primers.
- Allows the detection of aberrant species in your plasmid preparation (such as dimers, trimers, etc.).
- Gives you the backbone sequence of your plasmid every time.
- Does not produce poor-quality regions (such as the beginning and end of Sanger-type sequencing reactions).
What's more, our service also gives you an identified map of your plasmid (using the excellent pLannotate tool).
- 20 ul of plasmid at 40 ng/ul. *The plasmid must have been purified on column using a commercial kit. If purification has been carried out using an in-house protocol, sequencing results may be poorer or fail completely.
- A completed form containing some information about your samples (you can download the template here).
- Reference sequence (optional). If supplied, it will only be used to align the produced consensus sequence to your reference sequence, and to adapt (rotate and/or reverse complement) the consensus sequence, which will make your task easier during analysis.
- A consensus sequence (.fasta).
- A file containing statistics for each base of the consensus sequence (.xlsx).
- Two graphs reporting the quality and size of reads observed during sequencing (.png).
- An annotated map of the consensus sequence that can be viewed in a web browser (.html) or with the help of software such as snapgene or snapgene-viewer (.gbk).
- An alignment of the consensus sequence with your reference sequence (.aln, if provided). This alignment can be conveniently visualized using snapgene or snapgene-viewer software.