Splice Screen
A unique CRISPR/dCas13Rx platform that tiles guide RNAs across any transcript to map the RNA regulatory elements controlling splicing, stability, and expression.
The CRISPR/dCas13Rx Splice Screen is a unique functional genomics platform developed at the Sherbrooke RNA Platform. It enables high-throughput interrogation of RNA regulatory elements directly at the transcript level, revealing the cis-regulatory sequences that control how transcripts are spliced, stabilized, and expressed.
What You Can Study
Interrogate RNA regulation wherever it happens, from splice choices to transcript stability.
Exon Inclusion & Skipping
Find the cis-regulatory sequences that promote or repress exon inclusion in your transcript.
Intron Retention
Detect elements that control intron retention, a key layer of post-transcriptional regulation.
Alternative Splice-Site Choice
Map the sequences that steer selection between competing splice sites.
Variant Functional Impact
Determine whether missense, synonymous, or deep intronic variants act through RNA processing rather than protein sequence alone.
Gene-Expression Modulation
Target 3′UTR and other regulatory elements to tune RNA stability, degradation, and translation.
Therapeutic Target Discovery
Surface candidate targets for RNA-based interventions such as antisense oligonucleotides (ASOs).
How It Works
A systematic, transcript-level screen from design to regulatory map.
Tile guide RNAs across the transcript
We systematically tile CRISPR/dCas13Rx guide RNAs across the exons, introns, UTRs, and splice junctions of your gene of interest.
Perturb at the RNA level
dCas13Rx acts on the target RNA without altering the underlying genome, so function is probed directly at the transcript.
Read out regulatory effects
High-throughput readouts quantify how each guide shifts exon inclusion, splice-site use, intron retention, or transcript abundance.
Generate regulatory maps
The output is a cis-regulatory map of your transcript and a shortlist of candidate therapeutic targets.
Why Splice Screen
An innovative, scalable, and customizable solution for RNA regulatory research.
High-Throughput
Systematic tiling across the entire transcript in a single screen.
Transcript-Level Resolution
Functional readouts directly at the RNA, not inferred from DNA or protein.
Variant-Aware
Dissect the RNA-level impact of missense, synonymous, and deep intronic variants.
Customizable
Designed around your gene, your regions of interest, and your biological question.
Any Gene of Interest
Applicable to virtually any transcript you want to understand or manipulate.
Academic & Industrial
A scalable solution for academic research and industrial programs alike.
Have a Transcript to Decode?
Tell us your gene and your question, we'll design a Splice Screen that maps the RNA regulation you care about.