qRT-PCR
Complete gene expression assay workflow from target selection to relative expression analysis.
The qRT-PCR service is a complete gene expression assay workflow from target selection, primer design and validation, to qRT-PCR run, and relative expression analysis.
Key Features
SYBRgreen Technology
Quantitative RT-PCR using SYBRgreen fluorescence technology.
Bio-Rad CFX Systems
Bio-Rad CFX RealTime System: 384-plate (three units) and 96-plate (one unit).
Primer Validation
Primer validation using standard curve and target specificity verification by capillary electrophoresis.
High Throughput
5,000 reactions daily throughput for fast turnaround.
Pre-validated Primers
Growing database of pre-validated primers for common targets.
Available Applications
Gene Expression
Gene expression using qBase and Genorm methods (Hellemans 2007; Vandesompele 2002). Expression targets include: global, isoform-specific, sequence-specific or non-coding RNA.
miRNA Expression
miRNA expression using stem-loop method (Chen 2005).
Telomere & TREC
Telomere and TREC DNA quantification using Dion et al. method.
Pricing
| Number of Reactions | UdeS | Canadian Academic |
|---|---|---|
| 1–48 | $5.00 | $6.10 |
| 49–96 | $4.00 | $4.88 |
| 97–182 | $3.00 | $3.66 |
| 183+ | $2.00 | $2.44 |
- For academics external to Canada and industrials please contact us for an accurate quote.
- Not including cost for sample preparation and PCR primer design.
- Prices subject to change.
Ready to Start Your Project?
Contact us today to discuss how our qRT-PCR services can support your research.