Plasmid Sequencing
High-quality whole plasmid sequencing using Oxford Nanopore Technologies at $15 CAD/plasmid (UdS price).
Using a third-generation sequencing method (Oxford Nanopore Technologies (ONT)), our service enables high-quality sequencing of your whole plasmid(s).
How It Works
Create an account
Sign up on our plasmid sequencing portal. You will receive a confirmation email from noreply.rnomics@gmail.com (check your spam folder if needed).
Account activation
Once your account is approved, you will receive a confirmation email and can start submitting samples.
Submit your samples
Samples are sequenced in our next scheduled run (every Monday and Thursday).
Access your results online
You will receive an email notification as soon as your sequencing results are available on the portal.
This service is for you if:
- You have a long insert to sequence (requiring several Sanger sequencing reactions).
- You own or have received a plasmid for which you do not know the sequence.
- You want to check that a plasmid you've been using for a long time doesn't have unexpected mutations in its backbone.
Not suitable if:
- You're interested in a small insert (<500 bp).
- You have multiple amplicons in your samples (whether completely different or two alleles of a genome, e.g., for CRISPR).
Schedule
We perform sequencing runs every Monday and Thursday. Samples must absolutely be received by Monday or Thursday before 9 AM (both the portal submission and the physical tubes) to be included in that day's run, otherwise they will be included in the next run. Contact us if you have any questions about scheduling.
- Does not require specific primers.
- Detects aberrant species in your plasmid preparation (dimers, trimers, etc.).
- Always gives you the backbone sequence of your plasmid.
- No poor-quality regions (unlike Sanger sequencing), though long homopolymer stretches may be less accurate.
Our service also gives you an annotated map of your plasmid (using the excellent pLannotate tool).
- 20 uL of plasmid at 40 ng/uL. The plasmid must have been purified on a column using a commercial kit.
- A completed submission on our web portal.
- Reference sequence (optional).
- A consensus sequence (.fasta).
- A file with statistics for each base of the consensus sequence (.xlsx).
- A chromatogram-like web report for the consensus sequence (.html).
- Two graphs reporting quality and read lengths (.png).
- An annotated map viewable in a web browser (.html) or with SnapGene (.gbk).
- An alignment with your reference sequence (.aln), if provided.