ddPCR
Absolute nucleic acid quantification with the Bio-Rad QX200 Droplet Digital PCR system.
Digital droplet PCR (ddPCR) is a robust and highly sensitive nucleic acid quantification technique. This method enables absolute and direct quantification of gene expression, detection of rare mutations or SNPs, and copy number variation (CNV) analysis. We use the Bio-Rad QX200 Droplet Digital PCR system.
How ddPCR Works
This technology is based on partitioning the sample into approximately 20,000 nanoliter-scale droplets. Within each droplet, an end-point PCR reaction occurs on a single copy of the DNA template. The emitted fluorescence signal is read droplet by droplet (single or dual channel) to classify droplets as positive or negative based on a defined threshold. Target concentration is then inferred using a Poisson statistical model with 95% confidence intervals.
Available ddPCR Applications
Relative Expression Quantification
Quantify a target across different sample conditions with quadruplex reference gene normalization.
Absolute Quantification
Direct absolute quantification of different targets in a sample condition.
Mutation & SNP Detection
Detect mutations or SNPs against wild-type with high sensitivity.
Custom Applications
Other applications welcome for development and deployment at the platform.
Pricing
Per 8 Reactions
- For industrials please contact us for an accurate quote.
- Not including cost for sample preparation and PCR primer and hydrolysis probe design if applicable.
- Prices subject to change.
Ready to Start Your Project?
Contact us today to discuss how our ddPCR services can support your research.